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Erythritol is a four-carbon poly-hydric alcohol (C4H10O4) that exists naturally in a wide variety of foods and used as an alternative sweetener. Candida magnoliae isolated from honeycomb is an osmophilic yeast able to produce erythritol. Since the composition of growth medium is a crucial factor for erythritol production, optimizing the medium components was undertaken for C. magnoliae SR101, an osmophillic mutant of C. magnoliae. First, the effects of medium components were evaluated. Elimination of MgSO4 from the fermentation medium resulted in a 39% increase in erytrhritol productivity and a 38% increase in erythritol yield. Second, addition of 1 mg/L MnSO4 · H2O which is known to increase the permeability of a cell improved erythritol productivity by 6% and yield by 9% while the intracellular erythritol decreased from 0.02 g/L · DCW to 0.007 g/L · DCW. Addition of 5 mg/L of thiamine to the fermentation medium resulted in a 15% increase in productivity and 18% increase in yield