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영어

Recently, microbial fatty acids and their derivatives have been receiving increasing attention as an alternative source of fuel. The goal of the present study was to enhance the content of intracellular long-chain fatty acids in two bacterial strains, Pseudomonas aeruginosa PA14 and Escherichia coli K-12 MG1655, by co-overexpressing essential enzymes that are involved in the fatty acid synthesis metabolic pathway. By introducing two genes (accA and fabD) of P. aeruginosa into the two bacterial strains, and by co-expressing with them the fatty acyl-acyl carrier protein thioesterase gene of Streptococcus pyogenes, we have engineered recombinant strains that are efficient producers of longchain fatty acids. The recombinant strains exhibit a 1.3-1.7 fold increase in the production of long-chain fatty acids over the wild-type strains. To enhance the production of total long-chain fatty acids, were researched the carbon sources for optimized culture conditions and the results were used for post-culture incubation period. E. coli SGJS17 (containing the accA, fabD, and thioesterase genes) produced the highest content of total fatty acids; in particular, the unsaturated fatty acid content was about 20–fold higher than that in the wild-type E. coli.