earticle

영어

Butyric acid is a 4-carbon short chain fatty acid. It is widely used in chemical, food, and pharmaceutical industries. Generally, butyric acid is produced by Clostridium species that are strict anaerobes. Therefore, cultivation and genetic engineering are difficult with those strains. On the other hand, Escherichia coli is well-characterized microorganism and various genetic tools are available. The butyrate synthetic pathways of Clostridium acetobutylicum was constructed in recombinant E. coli. However, synthetic pathway constructed from exogenous enzyme often suffers from flux imbalance or intermediate loss. Therefore, we built synthetic protein scaffold that spatially co-localize enzymes. Scaffold protein may prevent the loss of intermediates and can effectively increase catalytic efficiency. Also, to study important factors for improving the productivity of butyrate, pta1, ldhA, frdA and adhE genes were deleted in E. coli and the effects of these mutations were compared