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포스터 발표 : 유전자 및 대사공학

Phosphorylation of chloramphenicol by a recombinant protein Yhr2 from Streptomyces avermitilis MA4680

원문정보

Thangamani RAJESH, Jong-Min JEON, Hyung Joo KIM, Sang Hyun LEE, Changmin SUNG, Byung-Gee KIM, Yung-Hun YANG

한국생물공학회 한국생물공학회 학술대회 2012 춘계학술대회 및 국제심포지움 2012.04 pp.185-185
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

Although phosphorylation of chloramphenicol has been shown to occur in chloramphenicol producer, Streptomyces venezuelae, there are no reports on the existence of chloramphenicol phosphorylase in other Streptomyces species. In the present study, we report the modification of chloramphenicol by a recombinant protein designated as Yhr2 from Streptomyces avermitilis MA4680. Recombinant Yhr2 was expressed in Escherichia coli BL21 (DE3) and the cells expressing this recombinant protein was shown to phosphorylate chloramphenicol to a 3’-Ophosphoryl ester derivative resulting in an inactivated form of the antibiotic. Expression of yhr2 conferred chloramphenicol resistance to E. coli cells upto 25 mg/mL. This study highlights that antibiotic resistance conferring genes could be easily expressed and functionalized in other organisms that does not produce the respective antibiotic.

키워드

저자정보

  • Thangamani RAJESH Dept. of Microbial Engineering, Konkuk University, Seoul.
  • Jong-Min JEON Dept. of Microbial Engineering, Konkuk University, Seoul.
  • Hyung Joo KIM Dept. of Microbial Engineering, Konkuk University, Seoul.
  • Sang Hyun LEE Dept. of Microbial Engineering, Konkuk University, Seoul.
  • Changmin SUNG School of Chemical and Biological Engineering, Seoul National University.
  • Byung-Gee KIM School of Chemical and Biological Engineering, Seoul National University.
  • Yung-Hun YANG Dept. of Microbial Engineering, Konkuk University, Seoul.

참고문헌

자료제공 : 네이버학술정보

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