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영어

The fixation of carbon dioxide is one of the most important issues for global sustainability. Formate dehydrogenase could be used for direct reduction of carbon dioxide to formate. Most formate dehydrogenases used in formate production were selenium-dependent and were not industrially feasible due to rapid inactivation upon exposure to oxygen. Recently, NAD-dependent formate dehydrogenases were utilized for reduction of carbon dioxide with advantages of oxygen stability, easy recombinant expression. Formate dehydrogenase from Candida boidinii (CbF) is commercially available and is most widely used. The reduction of carbon dioxide requires proton and reduction reaction at acidic pH is more efficient than at neutral pH. However, commercial CbF showed low reduction efficiency at acidic pH and it is necessary to look for efficient formate dehydrogenases for reduction of carbon dioxide at acidic pH. In this study, reduction activity of five formate dehydrogenases with acidic optimum pH was investigated. Four formate dehydrogenases showed higher reduction activity than the CbF and most efficient formate dehydrogenase had 3.2, 3.3, and 4.4 times higher reduction activity than the CbF at pH 5.5, 6.0, and 7.0, respectively. Further discussion will be presented.