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포스터 발표 : 효소공학

Characterization of carbonic anhydrase from Duneliala sp. and its application for CO2 sequestration.

원문정보

Seung Pil PACK, Bashistha Kumar KANTH, Ki Ha MIN, Shipra KUMARI, Ki Baek YEO, Eui Kyoung JANG, Mi-Ran KI

한국생물공학회 한국생물공학회 학술대회 2012 춘계학술대회 및 국제심포지움 2012.04 pp.175-175
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

Carbonic anhydrases (CAs) are zinc-containing metallo-enzymes that catalyze the inter-conversion of carbon dioxide and bicarbonate. The α -class CAs are found predominantly in vertebrates, but they are also found in Bacteria, algae and cytoplasm of green plants. α-CA from Duneliala sp (DspACA) shows to share high sequence homology to Dunaliella salina (dCAI). The codon optimized DspACA was cloned, expressed in E. coli BL21DE3, purified and characterized. The optimal induction concentration of IPTG and growth temperature was found to be 1.0 mM and 20oC.The optimum temperature of enzyme activity is 350C and pH 7.6 and 10.0. Apparent Km, Vmax and were 0.9095 mM, 3.303 x 10-8 mM min-1 for p-nitrophenylacetate substrate, respectively. Inhibitory effect on CA activity were determined using the esterase method under in vitro conditions. The inhibitory activity metal ions was in the order Fe2+ >Al3+ >Ag+ >Hg2+ >Cu2+ >Pb2+ >EDTA. Some metal ions show maximum enhancement in activity: Co2+ >Cd2+ >Zn2+. Acetazolamide shows maximum inhibition in the order : Acetazolamide >Sulphonamide >CN- >SCN- >F- >NO2- >Cl- >NO3- >SO42- >I- >Acetate.

키워드

저자정보

  • Seung Pil PACK Dept. of Biotechnology and Bioinformatics, Korea University, Chungnam, 339-700
  • Bashistha Kumar KANTH Dept. of Biotechnology and Bioinformatics, Korea University, Chungnam, 339-700
  • Ki Ha MIN Dept. of Biotechnology and Bioinformatics, Korea University, Chungnam, 339-700
  • Shipra KUMARI Dept. of Biotechnology and Bioinformatics, Korea University, Chungnam, 339-700
  • Ki Baek YEO Dept. of Biotechnology and Bioinformatics, Korea University, Chungnam, 339-700
  • Eui Kyoung JANG Dept. of Biotechnology and Bioinformatics, Korea University, Chungnam, 339-700
  • Mi-Ran KI Dept. of Biotechnology and Bioinformatics, Korea University, Chungnam, 339-700

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자료제공 : 네이버학술정보

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