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논문검색

포스터 발표 : 효소공학

Glycosylation of amentoflavone by recombinant oleandomycin glycosyltransferase from Streptomyces antibiotcus

원문정보

Yoon Ju JO, Se Eun YUN, Sung Hee CHOI, Eun Yeol LEE

한국생물공학회 한국생물공학회 학술대회 2012 춘계학술대회 및 국제심포지움 2012.04 pp.174-174
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

Glycosyltransferase can convert lipophilic secondary metabolites such as flavonoids, alkaloids, and terpenoids into glycosylated forms. The bioavailability of secondary metabolites can be improved by glycosylation. An oleandomycin glycosyltransferase (OleD GT) gene from Streptomyces antibioticus was functionally expressed in Escherichia coli BL21 (DE3). The purified recombinant OleD GT was employed as the biocatalyst for the glycosylation of amentoflavone as the acceptor via the transfer of glucose moiety from uridine-diphosphate-glucose (UDP-glucose) as the donor. The glycosylation reaction conditions were optimized and the time-course of the glycosylation of amentoflavone was analyzed. Amentoflavone was successfully converted into the corresponding glycosides by OleD GT-catalyzed batch glycosylation reaction.

키워드

저자정보

  • Yoon Ju JO Department of Chemical Engineering, Kyung Hee University, Gyeonggi-do 446-701, Korea.
  • Se Eun YUN Department of Chemical Engineering, Kyung Hee University, Gyeonggi-do 446-701, Korea.
  • Sung Hee CHOI Department of Chemical Engineering, Kyung Hee University, Gyeonggi-do 446-701, Korea.
  • Eun Yeol LEE Department of Chemical Engineering, Kyung Hee University, Gyeonggi-do 446-701, Korea.

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자료제공 : 네이버학술정보

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