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논문검색

포스터 발표 : 미생물공학

Cloning and Functional Expression of Carbon Monoxide Dehydrogenase from Citrobacter amalonaticus Y19 in E. coli

원문정보

Balaji SUNDARA SEKAR, Jungeun LEE, Eunhee SEOL, Ritam SARKAR, Sunghoon PARK

한국생물공학회 한국생물공학회 학술대회 2012 춘계학술대회 및 국제심포지움 2012.04 pp.167-167
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

Industrialization has resulted in emission of large amount of carbon monoxide into the atmosphere, which is hazardous to living organisms. Therefore conversion of CO to non toxic materials is highly essential. Hydrogen production from CO will be a value added contribution in replacing fossil fuels, as hydrogen has enormous potential as future energy due to its abundance and non-polluting nature. Citrobacter amalonaticus Y19, a facultative anaerobic bacterium, isolated by Jung et al. (2002) [1], was characterized for hydrogen production from the toxic carbon monoxide gas. Contig genome analysis of this microorganism revealed that cooFSCTJhypB genes are involved in expressing functional carbon monoxide dehydrogenase (CODH). These genes were heterologously overexpressed in E.coli BL21 (DE3) by cloning in pACYCDuet vector. This resulted in an enzyme activity of 269 μmol CO min-1mg protein-1, which is comparatively higher than the CODH activity in wild type Y19 [2].

키워드

저자정보

  • Balaji SUNDARA SEKAR Department of Chemical and Biochemical Engineering, Pusan National University, Busan 609-735, Republic of Korea.
  • Jungeun LEE Department of Chemical and Biochemical Engineering, Pusan National University, Busan 609-735, Republic of Korea.
  • Eunhee SEOL Department of Chemical and Biochemical Engineering, Pusan National University, Busan 609-735, Republic of Korea.
  • Ritam SARKAR Department of Chemical and Biochemical Engineering, Pusan National University, Busan 609-735, Republic of Korea.
  • Sunghoon PARK Department of Chemical and Biochemical Engineering, Pusan National University, Busan 609-735, Republic of Korea.

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자료제공 : 네이버학술정보

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