원문정보
초록
영어
Cellulosic materials of plant origin are the most abundant utilizable biomass resource mainly made up of pentose and hexose sugars. In this study, an alcoholic fermentation from hemicellulosic substrate composed of pentose was carried out by recombinant Pichia pastoris expressing functional minicellulosome comprising scaffolding protein and hemicellulase. To increase hydrolysis efficiency, P. pastoris was engineered for the assembly of minicellulosome by heterologous expression of a recombinant scaffolding protein mCbpA and xylanase XynB from Clostridium cellulovorans. The resulting strain could degrade hemicellulosic substrate efficiently with synergic enzyme reaction. In the fermentation of hemicellulosic materials, approximately 3.87 g/L ethanol was produced and it was 1.3 fold higher than that of wild-type strain. This recombinant yeast strain was useful for direct ethanol production from hemicellulosic substrate with synergic effect of the hemicellulolytic cellulosome. Enhanced ethanol productivity was achieved at expressing both of scaffolding protein mCbpA and xylanase XynB. It was concluded that recombinant P. pastoris is very suitable for industrial ethanol production from hemicllulose.