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Clostridium beijerinckii NCIMB 8052 as a butanol producer contains plenty of hydrolase and is able to utilize wide spectrum of carbohydrates. A 1,3-β-D-glucanase gene, which is annotated as Cbei_2828, was cloned from Clostridium beijerinckii NCIMB 8052 and expressed and purified in Escherichia coli BL21 (DE3). This glucanase gene has 1,362 base pairs and encodes a protein of 410 amino acids with a calculated molecular mass of 45 kDa. Signal peptideof this hydrolase was not cloned, so the total expressed protein has a molecular mass of 42 kDa. The encoded protein comprises a catalytic domain of glycosyl hydrolase family 16, fibronectin type 3 domain and Chitin/cellulose binding domain. The expressed enzyme was characterized. The activity of the expressed protein was tested by zymography and TLC by incubating the crude enzyme in Tris-Cl buffer containing 1% laminarin (w/v). The optimum working temperature and pH, substrate specificity and thermal stability of the purified enzyme will be tested. Kinetic parameters such as Km value and Vmaxwill also be evaluated.