earticle

영어

Ultraviolet-B (UVB) irradiation causes DNA damage and induces increase of matrix metalloproteinases (MMPs) in keratinocyte cells. Libanoridin is a natural product isolated from Corydalis heterocarpa which has recently been found to be effective antioxidant and anti-inflmmation. In this study, we assessed the effect of libanoridin aggainst UVB-induced damage using human keratinocyte (HaCaT) cells. In the results observed that libanoridin inhibited cell cytotoxicity and DNA damage due to 40 mJ/cm2 UVB exposure. Moreover, type IV collaganases MMP-2,-9 expression were decreased and MMP tissue inhibitor-1 (TIMP-1) decrease was inhibited followed by libanoridin treatment. In additionally we indicated that UVB-induced activation of phosphorylation of three MAPKase JNK, ERK, p38 and AP-1 transcription factor were decreased with libanoridin treatment. In conclusion, this study demonstrates that libanoridin has abilities to UVB induced cellular damage via MAPK and AP-1 signalling pathways, therefore, libanoridin may be used as an effetive natural compound on skin damage due to UVB exposure.