원문정보
초록
영어
Caffeine, aspirin, and tetracycline have been used in human life as diuretic, analgesic, and antibiotic. These pharmaceuticals have been detected in surface water resulting in the aquatic ecosystems confusion and human health change. In this study, it was analyzed specific stress responses caused by pharmaceuticals such as caffeine (890mg/l to 2225mg/l), aspirin (620mg/l to 1550mg/l) and tetracycline (180mg/l to 450mg/l) using four different stress responsive genes in Escherichia coli, i.e. fabA, grpE, katG and recA. The expression levels of these genes were quantified by semi-quantitative reverse transcription-PCR. It was found that four genes have shown different responsive patterns when E. coli cultures were under stressful conditions caused by caffeine, aspirin, tetracycline, respectively. Therefore, in this study, the stress responsive effects caused by these pharmaceuticals and the extent of each stress response can be analyzed using the expression levels and patterns of different stress responsive genes