earticle

영어

This study presents the effects of micro-geometries on the swimming behavior of Pseudomonas aeruginosa. First, we have measured parameters of single-cell motility including cell speed, run duration time, and tumble angle under two dimensional space. Recently the modern microfabrication tools allow us to create complex environments at the micrometer-scale and study of small micro-geometries. In the last decade microfabrication and microfluidics have been found to be very useful in the study of the motility of microorganisms. The results are used to calculate motility coefficients in the width of microchannels ranging from 10 to 100 μm. Since the single-cell motility parameters measured depend on the interaction of flagella with the microchannel wall, the duration time of the running cell in restricted geometries is distinctively different. Therefore, the motility of bacteria is decreased by restricted geometries. This study suggests that microfluidic approach is useful tool for the analysis of bacterial motility under the restricted space and rapid analytical tool.