원문정보
초록
영어
3-hydroxypropionic acid (3-HP), an industrially important platform chemical is used as a precursor for many commercially important chemicals. Recently, the production of 3-HP from glycerol was demonstrated in recombinant K. pneumoniae without the addition of expensive coenzyme B12 [1]. 3-HP production in K. pneumoniae is always associated with the production of 1,3-propanediol and lactic acid due to the generation of large amounts of NADH [2]. To enhance 3-HP yield on glycerol by regenerating NAD+, nitrate was supplemented in the culture medium. But this resulted in significant decrease in 3-HP production and majority of carbon was utilized for cell biomass generation, due to the presence of undesirable anaerobic respiration pathway. To overcome this limitation, a novel recombinant strain, Klebsiella pneumoniae DdhaTDglpK (puuC) was developed. This strain significantly increased 3-HP production at a maximum of 22.2 g/L in the presence of nitrate under anaerobic condition with a yield on glycerol at 0.35 mol/mol.