원문정보
초록
영어
Biodegradable cationic poly(β-amino esters) (PBAE) nanoparticles are promising tool for delivering therapeutic genes into defective tissues. PBAE nanoparticles not only can deliver gene non-virally but also mediate significant gene enhancement. Here, we demonstrated the delivery of therapeutic genes using amine-end modified PBAE nanoparticles into defective tissue model with skin wound. A prototypical morphogen called Sonic hedgehog (SHH) was applied as the therapeutic gene to heal and regenerate wounded area of skin. SHH is known to regulate various organ and tissue development during embryogenesis, but is also expected to promote tissue regeneration via angiogenesis. The complexes of SHH gene and PBAE nanoparticles were administered into full-thickness skin wound mouse model by intradermal injection. Wound closure of mice receiving PBAE-SHH gene delivery was accelerated compared to control group with intradermal delivery of b-galactosidase plasmid using PBAE nanoparticles. The quantitative real-time polymerase chain reaction assay and histological analysis revealed that there were significant improvement of epidermis regeneration and blood vessel formation in the PBAE-SHH group. In conclusion, SHH gene delivery using PBAE nanoparticles may be able to provide the potential gene therapy for wound healing.