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학생구두발표 (영문II), 좌장 : 양영헌 (건국대학교)

Co-production of 3-hydroxypropionic acid and 1,3-propanediol from glycerol using resting cells of recombinant Klebsiella pneumoniae J2B strain overexpressing aldehyde dehydrogenase

원문정보

Vinod KUMAR, Mugesh SANKARANARAYANAN, Kyeung-eun JAE, Meetu DURGAPAL, Somasundar ASHOK, Yeounjoo KO, Ritam SARKAR, Sunghoon PARK

한국생물공학회 한국생물공학회 학술대회 2012 춘계학술대회 및 국제심포지움 2012.04 pp.146-146
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

The co-production of 3-hydroxypropionic acid (3HP) and 1,3-propanediol (PDO) from glycerol was studied using the resting cells of a recombinant Klebsiella pneumoniae J2B strain [1] that overexpresses an aldehyde dehydrogenase. Active biomass was produced in M9 under a range of aeration conditions, and shifted to potassium phosphate buffer containing glycerol for bioconversion. The microaerobic or anaerobic conditions were favorable for both the production of active biomass and subsequent bioconversion. At flask level, the recombinant strain grown under microaerobic conditions produced 43.2 mM 3HP and 59.0 mM PDO from glycerol (117 mM) in 30 min with a cumulative yield of 0.87 (mol/mol). The fed-batch bioconversion performed in a 1.5 L bioreactor at a constant pH 7.0 under anaerobic conditions, resulted in 125.6 mM 3HP and 209.5 mM PDO in 12 h with a cumulative overall productivity, yield and maximum specific production rate of 27.9 mmol/L/h, 0.71 (mol/mol) and 128.5 mmol/g CDW/h, respectively.

키워드

저자정보

  • Vinod KUMAR Department of Chemical and Biomolecular Engineering, Pusan National University, Busan 609-735, South Korea.
  • Mugesh SANKARANARAYANAN Department of Chemical and Biomolecular Engineering, Pusan National University, Busan 609-735, South Korea.
  • Kyeung-eun JAE Department of Chemical and Biomolecular Engineering, Pusan National University, Busan 609-735, South Korea.
  • Meetu DURGAPAL Department of Chemical and Biomolecular Engineering, Pusan National University, Busan 609-735, South Korea.
  • Somasundar ASHOK Department of Chemical and Biomolecular Engineering, Pusan National University, Busan 609-735, South Korea.
  • Yeounjoo KO Department of Chemical and Biomolecular Engineering, Pusan National University, Busan 609-735, South Korea.
  • Ritam SARKAR Department of Chemical and Biomolecular Engineering, Pusan National University, Busan 609-735, South Korea.
  • Sunghoon PARK Department of Chemical and Biomolecular Engineering, Pusan National University, Busan 609-735, South Korea.

참고문헌

자료제공 : 네이버학술정보

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