earticle

논문검색

학생구두발표 (석사과정: 국문), 좌장 : 김선원 (경상대학교)

Inhibitory effect of Eisenia bicyclis extract on mRNA and protein expression of genes related in drug-resistance in methicillin-resistant Staphylococcus aureus cells

초록

영어

This study was performed to elucidate an antibacterial mechanism of Eisenia bicyclis extracts against methicillin-resistant Staphylococcus aureus (MRSA). Among of the extracts, the ethyl acetate soluble extract of E. bicyclis exhibited the highest anti-MRSA activity ranging in 128 μg/mL of minimum inhibitory concentration (MIC) against MRSA. Indeed, ethyl acetate extract was assessed its synergistic effects in combination with ß-lactam, ampicillin, penicillin and oxacillin against MRSA. Based on these results, we hypothesized that the ethyl acetate extraxct might inhibit a gene(s) or protein(s) related the drug resistance. It was observed that the ethyl acetate extract inhibited the mRNA expression of the resistant genes (mecI, mecR1 and mecA) in a dosedependent manner. Moreover, the results of Western blotting assays indicated that the ethyl acetate extract inhibited the synthesis of PBP2a protein. These results will provide a possible anti-MRSA mechanism by the ethyl acetate soluble extract of E. bicyclis.

저자정보

  • Jae-Hong PARK Dept. of Food Science and Technology, Pukyong National University, Busan 608-737.
  • Ji-Il CHOI Dept. of Food Science and Technology, Pukyong National University, Busan 608-737.
  • Yang-Bong LEE Dept. of Food Science and Technology, Pukyong National University, Busan 608-737.
  • Young-Mog KIM Dept. of Food Science and Technology, Pukyong National University, Busan 608-737.

참고문헌

자료제공 : 네이버학술정보

    함께 이용한 논문

      ※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.

      0개의 논문이 장바구니에 담겼습니다.