earticle

영어

Droplet-based microfluidic systems have emerged as a powerful platform for performing high-throughput biological experimentation. Fluorescence polarization(FP) measurements have been used to probe many biological reactions such as receptor-ligand binding, DNA hybridization, DNA-protein binding, peptide-protein binding, immunoassays and enzymatic degradation. FP detection is particularly advantageous since it is truly homogeneous assay format, allows for real-time (kinetic) measurements and is insensitive to concentration variations. For these reasons, we report herein the use of FP detection in a droplet-based microfluidic system.