earticle

영어

Osteoclasts are responsible for bone resorption on bone erosive surfaces in the marrow, which are key contributors to the bone pathogenesis of osteoporosis and rheumatoid arthritis. The induction of primary signals for osteoclast development and maturation depends on receptor activator of NF-κB ligand and macrophage-colony stimulating factor. Membrane bound signal adaptors such as FcRγ and DAP12 are also associated with osteoclastogenesis, supporting the cell surface expression of the activating-type immunoreceptors of the Ig superfamily or C-type lectin family. Anti-CLEC5A monoclonal antibodies to stimulate osteoclast formation were generated by rat immunization with mouse CLEC5A extracellular domain from E. coli and 5 antibodies showed their binding specificity with CLEC5A in western blot. Murine osteoclast differentiation model was established with RAW 264.7 cells to evaluate the antibody effect on osteoclastogenesis. Anti-CLEC5A monoclonal antibodies enhanced in vitro osteoclastogenesis and cell fusion as agonists and bound with U937 cells expressing human CLEC5A to suggest a potential cross-reactivity towards human CLEC5A.