earticle

영어

Skin homeostasis is interrupted during UV-A irradiation. How the UV-A altered skin components influences photoaging of skin should be investigated using human in vitro models that are important for understand skin aging. In the present study, chromene compound was isolated from Sargassum horneri and its potency inhibition of photoaging was investigated in UV-A irradiated dermal fibroblasts. Effects of compound on the prevention of photoaging were evaluated by measuring ROS production, membrane protein oxidation, lipid peroxidation and aging related gene expression in UV-A irradiated human skin dermal fibroblasts. The results indicated that treatment with compound suppressed the collagen degrading MMPs, MMP-1, MMP-2 and MMP-9 expression without any cytotoxicity and phototoxicity. It was further found that these inhibitions were due to increase in the expression of TIMP-1 and TIMP-2 genes. Furthermore, we confirmed that the UV-A induced transcriptions of AP-1 signaling pathway was regulated by compound treatment in UV-A irradiated dermal fibroblasts. Therefore, this study suggests that compound isolated from S. horneri can be developed as topical application of natural anti-aging cosmeceuticals as it reduced the skin collagen decomposition caused by UV-A irradiation.