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ecent development of microbial platforms for the production of bisabolene, a precursor of bisabolane, biosynthetic alternative to D2 diesel fuel, has been stimulated due to rising petroleum costs and environmental concerns (1). Microbial engineering based on Synthetic biology boosts the efforts to produce ‘Drop-In’ biofuels from renewable feedstocks. Synthetic biology aims to develop the foundational understanding and technologies to build intelligent biological systems to accomplish the breakthrough especially of metabolic engineering. In this presentation, I will present the IPTG-free bisabolene production system using engineered E. coli. Quorum-sensing (QS) mediated E. coli enables to induce neighboring cells as well as itself to initiate the carbon flux to the designated metabolic pathway without using expensive Isopropyl-b-D-1- thiogalactopyranoside (IPTG), a widely-used inducer, and showed the similar capability of genetic controls compared to the IPTG-induced system. Several genetic modules were constructed to link QS-system and biofuel pathway. Direct QS-system depending on cell density led the same level of bisabolene productions in E. coli. This QS-system for biofuels production could be a cost-effective and promising strategy to develop microbial platforms by reducing the usage of the exogenous inducer. (1) Peralta-Yahya et al., 2011 Nat. Commun.