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영어

The objective of this research was to investigate that NAADP can stimulate the differentiation of human keratinocytes and to build a foundation of the business based on the dermatological functions of NAADP.NAADP can stimulate to increase Ca2+ for cell signaling which synthesizes insulin from β-cell of pancreas, and now this signaling is very similar to the one for the differentiation of human keratinocytes. So we hypothesized that NAADP can stimulate the differentiation of human keratinocytes through cell signaling of Ca2+. To prove this hypothesis, we tested to increase Ca2+ and to express six kinds of differentiation marker in human keratinocytes by NAADP.As a result, Keratin1/10, Involucrin, Loricrin, Filaggrin, and Transglutaminase showed to be increased in western blot assay dependent on the concentration of NAADP, and also Keratin1/10, Filaggrin, and Loricrin showed to be increased by NAADP in immunohistochemistry by using hairless mice. Then we confirmed the function of NAADP for the differentiation of human keratinocytes. To investigate the efficacy of NAADP, Migration assay of human keratinocytes, repair assay of damaged human skin which induced by tape-stripping, moisturizing assay were carried out, and NAADP showed higher migration capacity than positive control group(growth factors), and excellent wound healing effect and moisturizing effect. When considered these results, we held a conviction for the business of NAADP, and wanted to build a foundation for this good substance, NAADP. So we have optimized the condition of NAADP production by enzyme reaction, and have done up-scale test for mass production. We concluded that NAADP can stimulate the differentiation of keratinocytes and give human skin the dermatological effects. If practice further studies of NAADP for signal transduction and genetic assay, we expect that NAADP will be valuable more and more.