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Generally, tissue-cultured plant has a benefit to solve the drawbacks such as a rareness, long time and high cost for growing herb. In this study, We have studied possibilities of applying cosmetic material about the two kinds of tissue culture obtained by using plant tissue culture techniques. One of these is a Anoectochilus formosanus. Anoectochilus formosanus, commonly known as "Jewel Orchids," which has been used in traditional folk medicines for fever, pain, and diseases of the lung and liver in Taiwan. The other one is the Echinacea angustifolia,.commonly known as compositae of dicotyledonous plant. It has been used in Indian traditional folk medicines for the treatment of disease like colds or other infections in North America. We artificially cultured Anoectochilus formosanus plantlet and Echinacea angustifolia adventitious roots by using the bioreactor culture system for this study. Previously, several studies have been reported on the efficacy (pharma –cological activities of lipid-metabolism, hepatoprotective activity, anti-tumor activity, neogenesis, wound healing, inflammation, hypertension, diabetes, liver disease, anti-tumor and immuno-stimulating effects) about these plants. Although these plants have been well known as herbs to remedy various diseases as a traditional medicine, we investigated whether the plantlet and adventitious roots cultured from these plants have efficacy as a cosmetic ingredient for skin care.