원문정보
초록
영어
An integrated allele-specific (AS) polymerase chain reaction (PCR) and resultant amplicon detection system has been developed for multiplex single nucleotide polymorphism (SNP) genotyping on a portable instrumentation, which was applied for on-site identification of HANWOO. As for the detection method, micro-capillary electrophoresis (CE) and microarray were employed, and the integrated PCR-μCE and PCR-microarray devices were constructed. Twelve sets of primers were designed for targeting eleven SNP loci and one primer set for a positive PCR control. The operation of microdevices were automated with a portable genetic analyzer, and the detection was performed on a miniaturized fluorescence detector for CE separation or a fluorescence scanner for microarray. Successful HANWOO verification was conducted with 99.37% fidelity in 90 min. Thus, our genetic analysis system provides rapid, accurate, and on-site multiplex SNP typing platform.