earticle

영어

In the past several decades, there have been significant advances in understanding of protein sequences, structures and functions which in turn have aided engineering of proteins with novel structures and/or functions. In addition, recent work from several laboratories has shown that an expanded repertoire of amino acid analogues can be incorporated into proteins. The recruitment of new amino acid constituents provides the protein engineering field with new tools and raises the prospects for creating novel proteins. In this talk, I will present a high-throughput screening method for engineering aminoacyl-tRNA synthetases to activate noncanonical amino acids and show examples how the engineered enzymes are utilized to study biology, especially to interrogate cellular protein synthesis.