earticle

영어

For the tissue engineering, the regulation of 3D microenvironment is important for the cell culture under in vivo like microenvironment and the study of cell pathophysiology, and it has been a great challenge by using conventional cell culture platform. Recent progress of microtechnology enabled the construction of microenvironment for the regulation of cellular behavior and differentiation. However, it is still difficult to build 3D in vivo mimicking microenvironments. Here, we propose the microscale arrayed concave PDMS wells for the formation and culturing of artificial cell spheroid in a easy and cost effective way and its use of 3D small tissue and further larger organ model. By the proposed technology, we created 3D micro tissue having uniform size and shape in a large scale using several kinds of cells including cancer cell, stem cell, neuron cell, hepatocyte and beta cells. In this presentation, we introduce the mass production of diverse cell spheroid: 1) embryoid body of mouse ES cell and their differentiation to neuron and cardiac cells, 2) hepatocyte spheroid, and hetero-spheroid including hepatocyte and hepatic stellate cell for the assisting liver function, and 3) islet spheroid production and in situ encapsulation with and collagen-alginate composite materials and in vivo evaluation of glucose control for 1 months. Finally, the new fibers which were coded with different chemicals or morphologies were fabricated using the microfluidic chip combined with computer controlled arrayed valve system. The applicability of these fibers to tissue engineering was evaluated through diverse cell culture in and on the fiber.