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논문검색

돼지 미성숙 난포란의 체외성숙에 미치는 Thymidine처리 효과

원문정보

Effect of Thymidine on In Vitro Maturation of Immature Porcine Follicular Oocytes

민성훈, 박흠대

피인용수 : 0(자료제공 : 네이버학술정보)

초록

영어

The objective of this study was to examine the effect of thymidine treatment during in vitro maturation (IVM) of porcine follicular oocytes on blastocyst development. Porcine oocytes were treated with thymidine (10 mM, 20 mM and 30 mM) for 2 or 6 hr in the preiods of IVM I and/or II. The survival rates of the blastocysts in the 6 hr treatment groups of 10 mM and 20 mM during IVM I period were significantly higher than those of control group (p<0.05). However, the survival rate of the blastocysts in the 2 hr treatment group of 20 mM during IVM II period was significantly higher than control group (p<0.05). Furthermore, the survival rate of the blastocysts in the 6 hr treatment group of 30 mM during IVM II period was significantly lower than control group (p<0.05). Consistent with the previous result, blastocyst development of both IVM I and II treatment group was also showed as similar pattern. Total and apoptotic cell numbers of blastocysts derived from thymidine treated porcine oocytes were examined by using Tunel assay. The results showed that there was no significant differences in total cell number of blastocysts between thymidine treated and untreated groups. However, apoptosis-positive cells in the thymidine treated group (6 hr IVM I) were significantly lower than those of other groups (p<0.05). Taken together, these results indicate that high quality oocytes were selected by DNA synthesis mechanism according to high concentration thymidine treatment during porcine oocyte maturation. Therefore, we concluded that presumptive selected oocytes by thymidine treatment during maturation periods improved the further embryo development and embryonic quality of IVF embryos by decreasing the incidence of apoptosis in preimplantation porcine embryos.

목차

ABSTRACT
 서론
 재료 및 방법
  배양액
  체외 성숙, 수정 및 배양
  TUNEL Assay
  실험 설계
 결과
  체외성숙 기간 동안 Thymidine 처리 효과
  Thymidine 처리에 의해 체외생산된 배반포의 질적 분석
 고찰
 인용문헌

저자정보

  • 민성훈 Sung-Hun Min. 대구대학교 생명공학과
  • 박흠대 Humdai Park. 대구대학교 생명공학과

참고문헌

자료제공 : 네이버학술정보

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