earticle

논문검색

연구논문

유기용매내성세균 Bacillus sp. BCNU 5006의 유용성

원문정보

Potential of Organic Solvent Tolerant Bacillus sp. BCNU 5006

최혜정, 황민정, 김봉수, 정영기, 주우홍

피인용수 : 0(자료제공 : 네이버학술정보)

초록

영어

In the screening process of organic solvent tolerant bacteria showing good growth in media containing several kinds of organic solvents, one strain was isolated and identified as Bacillus sp. BCNU 5006. The strain was able to tolerate many organic solvents including benzene, toluene, xylene, octane, dodecane, butanol and ethylbenzene. Likewise, it could also utilize these solvents as the sole source of carbon with significant enzyme production. The lipolytic enzyme stability of Bacillus sp. BCNU 5006 was studied in the presence of several kinds of solvents at a 25% (v/v) concentration. The highest enzyme stability was observed in the presence of octane (107%), followed by ethylbenzene (88%), decane (86%), and chloroform (85%). Especially, BCNU 5006 lipase was determined to be more stable than immobilized enzyme (Novozyme 435) in the presence of octane, chloroform and xylene. This organic solvent tolerant Bacillus sp. BCNU 5006 could be expected as a potential bioremediation agent and biocatalyst for biodegradation and provide on organicsolvent-based enzymatic synthetic method in industrial chemical processes.

목차

Abstract
 1. 서론
 2. 재료 및 방법
  2.1. 유기용매 내성 균주 분리
  2.2. 분리균주의 생리 ․ 생화학적 특성 및 동정
  2.3. 유기용매 내성 및 분해능 조사
  2.4. Lipase 생서능 조사 및 조효소액 조제
  2.5. Lipase 활성도 측정
  2.6. 효소의 온도 및 pH 안정성 조사
  2.7. 다양한 유기용매에 대한 효소의 안정성 조사
  2.8. 금속이온의 효소활성에 미치는 영향 조사
 3. 결과 및 고찰
  3.1. 유기용매 내성 세균 분리
  3.2. 생리 ․ 생화학적 특성 및 동정
  3.3. Bacillus sp. BCNU 5006의 유기용매 내성 및 분해능
  3.4. 유기용매 내성 세균이 생산하는 lipase의 다양한 온도와 pH에서의 lipase 활성
  3.5. Lipase의 안정성 및 활성에 미치는 유기용매의 영향
  3.6. Lipase 활성에 미치는 금속이온의 영향
 4. 결론
 References

저자정보

  • 최혜정 Hye Jung Choi. 창원대학교 생물공학협동과정
  • 황민정 Min Jung Hwang. 창원대학교 생물학과
  • 김봉수 Bong Su Kim. 창원대학교 생물학과
  • 정영기 Yong-Kee Jeong. 동아대학교 생명공학과
  • 주우홍 Woo Hong Joo. 창원대학교 생물공학협동과정, 2창원대학교 생물학과

참고문헌

자료제공 : 네이버학술정보

    함께 이용한 논문

      ※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.

      0개의 논문이 장바구니에 담겼습니다.