원문정보
Optimization of Agrobacterium-Mediated Transformation Parameters for Siberian Wildrye Grass Callus using GUS Gene as a Marker
초록
영어
Gene manipulation of Siberian wildrye grass through transgenic technology can modify grass feature for further improvement in the forage production. In order to optimize transformation conditions of Siberian wildrye grass, the effects of transformation efficiency was investigated with GUS gene. Seed-derived calli were infected and co-cultured with Agrobacterium tumefaciens carrying the binary vector pCAMBIA3301 encoding the GUS gene in the T-DNA region. The effects of several factors on transformation and the expression of the GUS gene were investigated. The highest transformation efficiency was obtained when embryogenic calli were inoculated with Agrobacterium strain EHA101 in the presence of 200 μM acetosyringone and coculture for 5 days. The present study was framed and materialized to standardize an efficient, high frequency and reproducible genetic transformation protocol for aromatic Siberian wildrye grass using Agrobacterium tumefaciens.
목차
Ι. 서론
Ⅱ. 재료 및 방법
1. 식물재료
2. Agrobacterium 배양 및 발현벡터
3. Agrobacterium 접종과 공동배양
4. 형질전환 효율과 캘러스 GUS 활성염색
Ⅲ. 결과 및 고찰
1. Acetosyringone 첨가농도에 따른 배양효율의 차이
2. Agrobacterium strain에 따른 배양효율의 차이
3. 공동배양 기간에 따른 형질전환 효율의 차이
Ⅳ. 요약
사사
Ⅴ. 인용문헌