원문정보
Expression of Oct-4 in Korean Native Cattle (Hanwoo) Germline
초록
영어
The Oct-4 (octamer-4), a member of the POU family transcription factor, is expressed in early mouse embryogenesis and in pluripotent embryonic stem (ES) lines. Oct-4 expression is thought to remain confined to the germline after gastrulation in the embryo. Therefore, the study was designed to, study the location of Oct-4 protein in the ovaries, placenta and testis of Korean native cattle (Hanwoo). Expression of Oct-4 mRNA in the ovaries and placenta of bovine was confirmed by RT-PCR and immunohistochemical analysis. Oct-4 was expressed in granulosa, thecal cells irrespective of the shape and size of follicles and endometerium of Korean native cattle (Hanwoo). Expression of Oct-4 was profound in all the tissues of Korean native cattle (Hanwoo) suggestung their role in them. Oct-4 localization and expression could contribute to further developmental studies in Korean native cattle (Hanwoo).
목차
Ι. 서론
Ⅱ. 재료 및 방법
1. 공시 동물
2. 공시 재료의 처리
3. RNA 추출 및 RT-PCR
4. 면역조직 염색법(immunohistochemistry)
Ⅲ. 결과 및 고찰
1. 한우의 태반, 난소 및 정소에서의 Oct-4 mRNA 발현
2. 한우의 난소와 태반에서의 Oct-4 단백질 발현 양상
Ⅳ. 요약
사사
Ⅴ. 인용문헌