원문정보
Isolation of a Marine-derived Flammeovirga sp. mbrc-1 Strain and Characterization of Its Agarase
초록
영어
A novel agar-degrading bacterium mbrc-1 was isolated from seashore of Kyungpo at Gangwon province and cultured in marine broth 2216 medium. Isolated bacterium mbrc-1 was named as Flammeovirga sp. mbrc-1 based on the 16S rDNA sequence. Its agarase showed maximum activity of 923 units/L at pH 7.0 and 45℃ and sustained 90% remaining activity after exposed to 45℃ for 2 hours. The enzyme hydrolyzed agarose to yield neoagarohexaose (18.5%), neoagarotetraose (38%) and neoagarobiose (43.5%), indicating that the enzyme is β-agarase. Thus, isolated bacterium and its β-agarase would be useful for the industrial production of neoagarotetraose and neoagarobiose.
목차
1. 서론
2. 재료 및 방법
2.1. 한천분해 균주의 분리 및 동정
2.2. 한천분해 균주의 생육 및 조효소액의 제조
2.3. 효소활성 측정
2.4. pH에 따른 한천분해 효소의 활성측정
2.5. 반응온도에 따른 한천분해 효소의 활성측정
2.6. 한천분해 효소의 열안정성 측정
2.7. 한천 가수분해산물의 thin-layer chromatography (TLC) 분석
3. 결과 및 고찰
3.1. 한천분해균주의 분리 및 배양
3.2. pH에 따른 한천분해 효소의 활성
3.3. 온도에 따른 한천분해 효소의 활성
3.4. 한천분해 효소의 열안정성
3.5. 한천 가수분해산물의 TLC 분석
4. 결론
References