원문정보
Isolation and Characteristics of Alginate lyase Producing Microorganism : Sanguibacter keddieii NC9
초록
영어
The research was purposed production of oligosaccharide from alginate hydrolysis the main composition in cell walls of sea weed. We was isolated 252 strains from sea water and mud flat, the highest alginate lyase activity was selected, and identified as Sanguibacter keddieii NC9 by 16S rDNA sequence analysis. In this study was select the sodium alginate concentration, pH, temperature for the production of alginate lyase activity. Alginate lyase activity was confirmed from plate assay with 10% cetylpyridinium chloride. The optimum culture conditions for the production of alginate lyase were sodium alginate 10 g/L, peptone 5 g/L, 40℃, pH 9 and 36 hours incubation time. Sanguibacter keddieii NC9, its alginate lyase would be useful for the production of bioenergy and biofunctional oligosaccharides from sea weed.
목차
1. 서론
2. 재료 및 방법
2.1. Alginate lyase 활성 균주 분리
2.2. 분리된 균주의 alginate lyase 활성 확인
2.3. Alginate 농도에 따른 효소활성 및 세포성장 측정
2.4. 배양조건별 균주의 성장 및 alginate lyase 활성 측정
3. 결과 및 고찰
3.1. Alginate lyase 생산 균주 분리 및 동정
3.2. Alginate 농도에 따른 균주 성장과 alginate lyase 활성
3.3. pH 및 온도에 따른 alginate lyase 활성
4. 결론
References