원문정보
초록
영어
Cellular uptake of nanoparticles for stem cell labeling and tracking is a critical technique for biomedical therapeutic applications. However, current techniques suffer from low intracellular labeling efficiency and cytotoxic effects, which has led to great interest in the development of a new labeling strategy. Using silica-coated nanoparticles conjugated with rhodamine B isothiocyanate (RITC) (SR), we tested the cellular uptake efficiency, biocompatibility, proliferation or differentiation ability with murine bone marrow derived hematopoietic stem/progenitor cells. The bone marrow hematopoietic cells showed efficient uptake with SR with dose or time dependent manner and also provided a higher uptake on hematopoietic stem/progenitor cells. Biocompatibility tests revealed that the SR had no deleterious effects on cell cytotoxicity, proliferation, or multi-differentiation capacities in vitro and in vivo. SR nanoparticles are advantageous over traditional labeling techniques as they possess a high level of cellular internalization without limiting the biofunctionality of the cells. Therefore, SR provides a useful alternative for gene or drug delivery into hematopoietic stem/progenitor cells for basic research and clinical applications.
목차
INTRODUCTION
MATERIALS AND METHODS
Preparation of Nanoparticles
Preparation of Hematopoietic Cells
Flow Cytometry Analysis
Cell Viability and Proliferation Assay
Cell Cycle Analysis
Colony-Forming-Cell Ability Assay
In Vivo Hematopoietic Reconstitution Activity
Statistical Analysis
RESULTS
Uptake Efficiency into Bone Marrow Hematopoietic Cells
Cell Proliferation and Cytotoxic Effects
In Vitro Hematopoietic Differential Effects
In Vivo Hematopoietic Reconstitution Effects
Intracellular Uptake into Hematopoietic Stem/Progenitor Cells
DISCUSSION
REFERENCES