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초록
영어
The experimental manipulation of protooncogenes and their gene products is a valuable research tool for the study of human neoplasia. In this study, the recently identified human cervical cancer protooncogene (HccR-2) was expressed in transgenic mice under the control of the tetracycline regulatory system. The phenotype observed was similar in many respects to human chronic neutrophilic leukemia (CNL). Thus, the HccR-2 transgenic mouse model is important not only for investigating the biological properties of the HccR-2 protooncogene in vivo, but also for analyzing the mechanisms involved in the progression of CNL.
목차
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Production of Transgenic Mice
PCR Analysis
Southern Blot Hybridization Analysis
RNA Analysis by Reverse Transcriptase-PCR (RT-PCR)
Western Blot Hybridization Analysis
Morphologic and Histologic Analyses
Statistical Analysis
RESULTS
Generation of the Transgenic Mice Expressing HccR-2
Peripheral Blood and Bone Marrow Features
Development of Chronic Neutrophilic Leukemia (CNL)
Leukemic Transgenic Mice were Susceptible to Infection
DISCUSSION
REFERENCES
INTRODUCTION
MATERIALS AND METHODS
Production of Transgenic Mice
PCR Analysis
Southern Blot Hybridization Analysis
RNA Analysis by Reverse Transcriptase-PCR (RT-PCR)
Western Blot Hybridization Analysis
Morphologic and Histologic Analyses
Statistical Analysis
RESULTS
Generation of the Transgenic Mice Expressing HccR-2
Peripheral Blood and Bone Marrow Features
Development of Chronic Neutrophilic Leukemia (CNL)
Leukemic Transgenic Mice were Susceptible to Infection
DISCUSSION
REFERENCES
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참고문헌
자료제공 : 네이버학술정보