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Purpose: The purpose of this study was to identify the relationship between spiritual well-being, depression and life satisfaction majoring in human service area. Methods: The subjects were 212 college students. Data was collected from september 15 to October 26, 2009 using questionnaires. Means with standard deviation, t-test, ANOVA, and Pearson correlation coefficients were used to analyze the data. Results: According to general characteristics of the students, there were statistically significant differences in spiritual well-being for religion and religious influence, in depression, there was statistically significant difference for health status, and in life satisfaction, there were statistically significant differences monthly pocket money, health status, current survival parents status, current problem. significant correlations were seen between spiritual well-being and depression (r=-.386, p =.000), life satisfaction (r=.536, p =.000). Conclusion: Spiritual well-being was significantly effects on depression and the life satisfaction. The finding suggest that it is needed to develop effective programs to improve spiritual well-being for college students.