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Purpose : The purpose of this study was to evaluate the effects of regular health education by school nurses on middle school student's health related knowledge, attitude and behavior. Methods : A quasi-experimental design with pretest and posttest measures was used. Questionnaire survey was conducted to middle school students(1st, 2nd year). The participants of the study were composed two groups : 274 in health education group and 336 in control group. Results : After seventeen-week of receiving regular health education, differences were observed between the experimental group and the control group to health knowledge(F=7.901, p=.005), health attitude(F=4.174, p=.042) and health behavior(F=7.675, p=.006). Conclusion : The regular health education by school nurses on middle school students improve their health related knowledge, attitude and behavior. So it is recommended to develop standardized manual and educational materials for providing systematic and effective health education.