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Purpose: This descriptive study was to examine the effect factors on health promotion lifestyle of shift work nurses. Methods: The 418 working nurses who completed questionnaires with consent were analysed. Results: The average level of health promotion lifestyle of shift work nurses was 2.21 and it was significantly lower than 2.36 of fixed work nurses. In shift work nurses, the highest health promotion lifestyle was personal relationship of 2.79 and the lowest was physical activity of 1.58. The young, shorter shift work duration, unmarried and general nurses were evaluated as not-healthy diet. The old, longer shift work duration and married showed less physical activity. Less self-efficacy and more job stress, depression, fatigue showed lower health promotion lifestyle. Conclusion: Self-efficacy on health behavior of shift work nurses was the most effective factor on all areas of health promotion. Therefore, we need to develop a health promotion program including self-efficacy encouragement. We suggest that this program be more effective when more precisely characterized according to age, marital status, shift work duration, job position, and psychological problems such as depression and fatigue.