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Purpose: The purpose of this study was to examine effects of smoking cessation program in large size company using motivational enhancement counseling. Methods: A quasi-experimental design was conducted. 72 workers in intervention group and 65 workers in control group participated in 7 large size companies. Smoking cessation program consisted of a campaign, smoking cessation education, and 6 weeks motivational enhancement counseling. The motivational enhancement counseling was tailored to the individual's stage of change. Stages of smoking behavior, smoking cessation rate, nicotine dependence, and confidence of smoking cessation were assessed after intervention. Results: The results showed that intervention had a significant impact on improving stages of smoking behavior and smoking cessation rate, and impact on decreasing nicotine dependence, compared to those of a control group. Conclusion: The effects and feasibility of smoking cessation program including motivational enhancement counseling were revealed in the large size company. These results might provide information that can be used in improving cultures of smoking cessation for workers.