earticle

영어

To evaluate the effect of Rosemary(Rosemarinus officinalis) extract on antioxidative activity and melanogenesis on cultured SK-MEL-3, the cell viability, DPPH-radical scavenging activity, superoxide dismutase(SOD)-like activity, tyrosinase activity and a total amount of melanin were measured. In this study, SK-MEL-3 cells were treated with the media containing 1∼20μM, hydrogen peroxide(H2O2) for 4 hours. H2O2 significantly decreased cell viability compared with control and XTT50 value was determined at 20μM of H2O2. In the antioxidative effect of Rosemary extract, it showed DPPH-radical scavenging activity and SOD-like activity. While, in the melanogenic effect of Rosemary extract, it significantly decreased the tyrosinase activity and a total amount of melanin which were increased by H2O2. From above the results, it is suggested that Rosemary extract was effective in the protection of H2O2-induced cytotoxicity by antioxidative activity and inhibition of melanogenesis.