원문정보
Enhancement of Saccharification Yield of Ulva pertusa Kjellman by High Pressure Homogenization Process for Bioethanol Production
초록
영어
This study was investigated to improve the saccharification yield of Ulva pertusa Kjellman by the high pressure homogenization process. It was found that the high pressure homogenization pretreatment effectively destructed the cell wall structures only by using water. The high pressure homogenization process was operated under various conditions such as 10000, 20000 or 30000 psi with different recycling numbers. The optimal condition was determined as 30000 psi and 2 pass of recycling numbers and the sugar conversion yields were 16.02 (%, w/w) of glucose and 14.70 (%,w/w) of xylose, respectively. In the case of enzymatic treating the hydrolyzates with 5 FPU/glucan of celullase and 100 units/mL of amyloglucosidase, 65.8% of carbohydrates was converted into glucose. Using the hydrolysates of Ulva pertusa Kjellman, 48.7% of ethanol was obtained in the culture S.cerevisiae. These results showed that the high pressure homogenization process could efficiently hydrolyze the marine resource by using only water for bioethanol production.
목차
1. 서론
2. 재료 및 방법
2.1. 실험재료
2.2. 방법
2.3. Scanning Electron Microscope (SEM) 및 Dynamic LightScattering (DLS)을 통한 구멍갈파래의 입자크기와 표면 관찰
2.4. 가수 분해 잔유물의 2차 효소처리
2.5. 전처리 부산물 내 HMF 농도 측정
2.6. 구멍갈파래 가수분해물의 에탄올 발효
2.7. 통계
3. 결과 및 고찰
3.1. 고압균질 공정을 이용한 구멍갈파래 전처리
3.2. 전처리 가수분해물의 효소당화 공정
3.3. 구멍갈파래 가수분해물 내 발효저해물질 농도 측정
3.4. 구멍갈파래 가수분해물의 에탄올 발효 공정
4. 결론
감사
References