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특집 : 식품효소 - 연구논문

김치 유래 유산균의 항균적 특성과 이용

원문정보

Chacteristics & Applications of Lactobacillus sp. from Kimchi

김기은

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초록

영어

Earlier in Korea Kimchi was made in every family and every province has own taste and specialties. These days almost of the Kimchis are manufactured. We collected variable Kimchis, which were made for private use and isolated microorganisms. Some interesting micobial cells were identified and studied for its application as food and drinks. One of them was identified as Lactobacillus sakei KJ123. This strain is known as producing interesting aromatic components during Sakei fermentation like Kimchi in variable conditions. We tried to develop a health beverage with fermentation process. The Cucurbita maxima has been known as a traditional healthy food and variable positive effects on the human body were already reported. In this study we tried to develop a production process for a healthy fermented drink on this substrate with strains originated from Kimchi. Many kinds of lacctobacilli species existed in the fermented food cannot survive in the acidic conditions like human stomach. So we selected resisting strains in this conditions. The survival rate of Lactobacillus sakei cells in the artificial gastric juice and bile acid and other physiological characteristics at the variable conditions have been tested. After fermentation process some sensory tests on the product with panels were tried.

목차

Abstract
 1. 서론
 2. 재료 및 방법
  2.1. 김치에서 유산균 분리
  2.2. 생육 특성 조사
  2.3. 인공위액에 대한 내산성균주 분리
  2.4. 단호박 배지 제조
  2.5. 단호박 배지에서 유산균의 생장곡선과 pH측정
  2.6. 유산균 단호박 배지 발효물의 항균활성 측정
  2.7. HPLC 분석
  2.8. pH, 적정산도 및 생균수의 측정
  2.9. 분리된 균주의 생장곡선
  2.10. 16s rRNA Full Sequencing
  2.11. 농도별, 발효시간별 단호박 발효음료의 관능검사
 3. 결과 및 고찰
  3.1. 김치에서 유산균 스크리닝
  3.2. 인공위액에 대한 내산성균주 분리
  3.3. 인공담즙산에 대한 내성균주 분리
  3.4. 16s rRNA Full Sequencing
  3.5. 단호박 배지에서 발효과정중의 OD, 포도당, pH값의 변화
  3.6. 유기산 분석
  3.7. 유산균 단호박배지 발효물의 항균활성 측정
  3.8. 농도별, 발효시간별 단호박 발효음료의 관능검사
 4. 고찰
 5. 결론
 References

저자정보

  • 김기은 Gi-Eun Kim. 서경대학교 생물공학과

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