원문정보
The Early Detection of the Spore Using Sonication and Fluorescent Dye in the Field
초록
영어
This study was carried out to establish the optimum condition of cell disruption with a sonicator for the detection of the spore, Bacillus anthracis Δ-sterne for the purpose of developing automatic fluorometer. The efficiency of sonication on the Δ-sterne spore disruption was very weak. The Δ-sterne spore with zirconia bead showed greater disruption than the Δ-sterne spore alone when sonificated. The volumn of the zirconia bead added in the spore solution has little effect on the disruption efficiency. The detection limit of the Δ-sterne spore with zirconia bead and the Δ-sterne spore alone was 106 CFU/mL and 5 × 107 CFU/mL respectively, when sample was sonicated for 20 seconds with a sonicator probe of 13 mm diameter.
목차
1. 서론
2. 재료 및 방법
2.1. 균주 및 재료
2.2. 생균수 측정 및 기기분석
2.3. 포자농도별 초음파 처리에 대한 형광량
2.4. Zirconia bead 처리에 대한 형광량
2.5. sonication 처리시간에 따른 형광량
2.6. 포자 탐지농도
3. 결과 및 고찰
3.1. 포자농도별 초음파 처리에 대한 형광량
3.2. Zirconia bead 처리
3.3. sonication 처리시간
3.4. 포자 탐지농도
4. 결론
References