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특집 : 줄기세포 조직공학 - 연구논문

이타콘산 고생산성 Aspergillus terreus 변이주의 신속 선별을 위한 효율적인 균주 스크리닝 전략 개발

원문정보

Development of an Efficient Screening Strategy for Rapid Selection of High-yielding Mutants of Itaconic Acid Biosynthesized by Fungal Cells of Aspergillus terreus

신우식, 김평현, 이도훈, 김상용, 정용섭, 전계택

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초록

영어

An efficient screening method was developed for rapid selection of a few overproducers of itaconic acid (IA) among the great many mutants derived from mother strains of Aspergillus terreus. For this purpose, an attempt was made to reveal the relationships of the growth rate and sporulation of each mutant on PDA solid medium with its IA productivity in the final liquid production-culture. As a result, it was possible to classify the mutated strains into 5 groups (from [A] to [E] group) according to theirmorphologies (i.e., growth rate and sporulation extent) on the PDA slants. Notably, most of the high-yielding mutants of IA were observed to belong to [A]group which had the properties of the highest growth rate and sporulation among the 5 groups, whereas the mutant groups of [C], [D] and [E] with the contrasting morphological features showed significant reductions in their IA productivities. From these results, it was concluded that the probability of selecting IA overproducing mutants could be remarkably enhanced when the mutated colonies showing faster growth rates are firstly selected on the PDA plate, and then further screening process is performed on the basis of the sporulation extents of the mutants selected. Consequently, through the application of the strategy developed in this study, costs and time involvedin the labor-intensive task of strain improvement could be reduced to a great extent, because the time-consuming liquid culture processes did not need to performed for the unfavorable mutants belonging to the groups other than group [A].

목차

Abstract
 1. 서론
 2. 재료 및 방법
  2.1. 균주 및 보관
  2.2. 배양 배지 및 배양 조건
  2.3. 균체 농도 및 이타콘산 정량분석
  2.4. 이타콘산 고생산성 우량 변이주 개발을 위한 NTG 돌연변이 유발 방법
 3. 결과 및 고찰
  3.1. 돌연변이주의 PDA plate고체배지에서의 성장속도와액상 생산배양에서의 이타콘산 생산성과의 상관관계(Aspergillus terreus NR38 균주를 모균주로 사용)
  3.2. 변이주의 고체배지에서의 성장속도 및 포자형성 정도가이타콘산 생산성에 미치는 영향 (Aspergillus terreus NR109균주를 모균주로 사용)
  3.3. 이타콘산 고생산성 변이주들의 PDA 고체배지에서의배양 특성 재조사 (Aspergillus terreus 1212.12 균주를 모균주로 사용)
 4. 결론
 References

저자정보

  • 신우식 Woo-Shik Shin. 강원대학교 의생명과학대학
  • 김평현 Pyeung-Hyeun Kim. 강원대학교 의생명과학대학
  • 이도훈 Dohoon Lee. 한국생산기술연구원
  • 김상용 Sangyong Kim. 한국생산기술연구원
  • 정용섭 Yong-Seob Jeong. 전북대학교 응용생물공학부
  • 전계택 Gie-Taek Chun. 강원대학교 의생명과학대학

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