원문정보
Effect of Magnetized Water on Cryopreservation in Bovine Spermatozoa
초록
영어
The purpose of this study was to improve of frozen-thawed sperm using magnetized water in Korean native cattle. Before cryopreservation, without egg-yolk Triladyl® solution was flowed though magnet [0, 2000, 4000 and 6000 Gauss(G)] for 5 min. The freezing of dilluted semen added with Triladyl containing 20% egg-yolk. Analysis of frozen-thawed sperm was estimated viability with SYBR14/PI double stain, membrane intact with hypoosmotic swelling test (HOST), acrosome reaction with FITC-PNA, mitochondria membrane function with Rhodamin 123 by flow- cytometry. Sperm viability was significantly higher in 4000G group than other groups (p<0.05). However, the Hypoosmotic Swelling Test(HOST) was significantly higher in fresh, 4000 and 6000G than 0 and 2000G (p<0.05). In addition, mitochondria membrane damage and acrosome damage were significantly lower in 6000G group than other groups (p<0.05). in conclusion we suggest that magnetized water could be improve ability of sperm on cryopreservation in Korean native cattle.
목차
서론
재료 및 방법
정액 채취
정액 동결
동결정액 융해 및 성상검사
생존율 검사(SYBR-14/PI Staining)
정자원형질막 기능 검사(Hypoosmotic Swelling Test)
미토콘드리아 검사(Rhodamin123/PI Staining)
첨체검사(FITC-PNA/PI Staining)
통계처리(Statistical Analysis)
결과
자기활성된 동결액 처리 후 생존율 평가
정자의 원형질막 검사(Hypoosmotic Swelling Test)
정자의 미토콘드리아 검사
자기활성된 동결액 처리 후 정자의 첨체 손상 검사
고찰
인용문헌