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PhosphoproteinEnrichedinAstrocytes15(PEA15)가 과발현하는 형질전환 돼지의 생산

원문정보

Production of a Transgenic Pig Overexpressing Phosphoprotein Enriched in Astrocytes 15 (PEA 15)

이휘철, 김현미, 이승훈, 오건봉, 정학재, 양병철, 김경운, 이풍연, 박진기, 장원경

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초록

영어

The overexpression of Phosphoprotein Enriched in Astrocytes (PEA15) gene is commonly found in human diabetic patients. The overexpression of this gene in skeletal muscle and fat tissues have been reported to cause insulin resistance, thereby impairing insulin stimulated glucose uptake. We introduced a gene of mouse PEA15 (mPEA15) and enhanced green fluorescent protein (EGFP) into fertilized one cell pig zygotes using microinjection, and produced a piglet that showed overexpression of mPEA15 in the muscle tissues and expression of EGFP in the ear tissues and hooves. RT-PCR RFLP, southern blot and FISH analysis showed that the tissues carried the transgene. Real-time RT-PCR and western blots demonstrated that PEA15 gene was overexpressed in the various tissues and muscle tissues, respectively. These facts suggest that expression vector system is normally expressed in the trnasgenic (TG) pigs. To use as animal diseases model for type 2 diabetes, further study is necessary to confirm whether diabetes occur in these TG pigs, especially insulin resistance.

목차

ABSTRACT
 서론
 재료 및 방법
  미세 주입용 유전자 준비
  Micropipets 제작 및 유전자 미세 주입
  외과적 방법에 의한 수정란 채란 및 이식
  RT-PCR RFLP 및 Real-time RT-PCR
  Southern Blot Hybridization
  Western Blotting
  Fluorescence In situ Hybridization (FISH)
 결과 및 고찰
  mPEA15의 과발현 및 EGFP 발현의 확인
 인용문헌

저자정보

  • 이휘철 Hwi-Cheul Lee. 농촌진흥청 국립축산과학원
  • 김현미 Hyun-Mi Kim. 농촌진흥청 국립축산과학원
  • 이승훈 Seunghoon Lee. 농촌진흥청 국립축산과학원
  • 오건봉 Keon-Bong Oh. 농촌진흥청 국립축산과학원
  • 정학재 Hak-Jae Chung. 농촌진흥청 국립축산과학원
  • 양병철 Byong-Chul Yang. 농촌진흥청 국립축산과학원
  • 김경운 Kyung-Woon Kim. 농촌진흥청 국립축산과학원
  • 이풍연 Poongyeon Lee. 농촌진흥청 국립축산과학원
  • 박진기 Jin-Ki Park. 농촌진흥청 국립축산과학원
  • 장원경 Won-Kyong Chang. 농촌진흥청 국립축산과학원

참고문헌

자료제공 : 네이버학술정보

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