원문정보
초록
영어
The purpose of this study was to develop an analytical method for determining 15 polycyclic aromatic hydrocarbons (PAHs) of EU priority using gas chromatography (GC)-tandem mass spectrometry (MS). The PAHs in ground coffee were analyzed after being extracted using methods such as saponification-liquid-liquid extraction, Soxhlet extraction, and solid-liquid extraction. The solid-liquid extraction method showed the greatest repeatability and most efficient reduction of the matrix effect. GC-tandem MS for the quantification of the 15 PAHs showed better resolution and lower limit of detections (LODs) than GC-MS-selected ion monitoring (SIM) and high performance liquid chromatography with fluorescence detector. LODs of this method for the ground coffee types were 0.002-0.1 μg/kg and limit of quantifications (LOQs) were 0.006-0.2 μg/kg The recoveries ranged from 52.6 to 93.3%. Forty-six commercial types of ground coffee were analyzed to determine their PAHs contamination levels. PAHs concentration ranged from ND to 5.988 μg/kg. This study was conducted with toxicity equivalence factors, the U.S. EPA recommendation to identify dietary risks for PAHs in different types of coffee. The estimated average daily dose of PAHs was 5.24×10−8 mg/kg body weight/ day.
목차
서론
재료 및 방법
재료 및 시약
장비
기기분석
초음파 후 고체-액체 추출
비누화 후 액체-액체 분배추출
Soxhlet 추출
정제
절대회수율(absolute recovery) 검토
원두커피 추출액으로의 이행계수
결과 및 고찰
원두커피중 PAHs 전처리 방법 비교 및 확립
절대회수율(Absolute recovery)
검량선작성, 검출한계 및 정량한계
시중 유통 원두커피의 PAHs 함량조사
인체노출량 평가
요약
문헌