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In this study, we carried out an experimentation with twelve college girl students for two weeks in the swimming pool of a condominium in Sokcho area. Ten times of experiment were made from November 29th to December 10th 2010 excepting weekends. In order to gauge the stress relieving effect of Watsu, density of salivary cortisol, systolic blood pressure, and diastolic blood pressure before and after watsu application were measured and analyzed in accordance with the three time periods, that is, before the experiment, five days after the experiment, and 10 days after the experiment. The following results were drawn from the experiment. 1. Change in the density of salivary cortisol before and after Watsu application was decreased and the degree of decrease was on the rise in a piecemeal way, which is supported by statistical significance. 2. In the comparison of the highest systolic blood pressure before and after the application of Watsu, gradual decreasing of the blood pressure was observed and supported by statistical significance. 3. Of the lowest diastolic blood pressure, no striking change was observed and statistical number was negligible.