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The purpose of this paper was to evaluate performance of information system for one national university hospital in order to identify the factors influencing performance of information system. KPIs were collected for 181 users of information system (41 doctors, 104 nurses, and 11 medical supporting staffs, and 25 administrative staffs) from August 10 to 24, 2010. The results were as follows: Average performance score for input layer was 3.16; average performance score for process layer was 3.35; and average performance score for business layer was 3.57. Scores for input layer was lowest for nurses and scores for process and business layer were lowest for doctors. Results from the path analysis showed that system quality, demographic characteristics, and security significantly influenced management process but these factors except demographic characteristics influenced user satisfaction; and management process also significantly influenced user satisfaction.