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초록
영어
Staphylokinase, a 163 amino aci d bacterial protein from Staphylococcus aureus is reported to have a therapeutic function and used as a potential alternative for the available clot dissolving drugs and some countries proved to be a potent alternative over the available clot dissolving drugs. In the present study, sak gene is isolated from a lysogenically converted S.aureus and expressed in non-pathogenic E.coli expression host. The sak gene is cloned into pET-28a vector and expressed in an IPTG and salt induced E. coli strain (GJ1158). The mature recombinant sak protein expressed in E.coli is also further extracted and analyzed by various methods to demonstrate thrombolytic activity.
목차
Abstract
1. Introduction
2. Materials and Methods
2.1 Microbial Stains
2.2 Culture Conditions
2.3 Isolation and Screening of Staphylokinase Producing Microbial Source
2.4 Identification and Cloning of Sak Gene from Isolated Sample
2.5 Expression and Activity Analysis of Recombinant SAK
3. Results and Discussion
3.1 Identification of Collected Sample
3.2 Screening for Staphylokinase Activity
3.3 Isolation of Gene Coding for SAK
3.4 Generation of Recombinant DNA
3.5 Expression and Activity Analysis
4. Conclusion
References
1. Introduction
2. Materials and Methods
2.1 Microbial Stains
2.2 Culture Conditions
2.3 Isolation and Screening of Staphylokinase Producing Microbial Source
2.4 Identification and Cloning of Sak Gene from Isolated Sample
2.5 Expression and Activity Analysis of Recombinant SAK
3. Results and Discussion
3.1 Identification of Collected Sample
3.2 Screening for Staphylokinase Activity
3.3 Isolation of Gene Coding for SAK
3.4 Generation of Recombinant DNA
3.5 Expression and Activity Analysis
4. Conclusion
References
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