원문정보
초록
영어
We examined the in vitro mutagenic and antimutagenic effects of hexane- extracted hemp (Cannabis sativa L. subsp. sativa var. sativa) seed oil (HO) with and without S9-mediated metabolic activation, using the TA98 and TA100 Salmonella Typhimurium strains. The MTT assay revealed no cytotoxicity in HepG2 cells for HO quantities <400 g/mL. In the mutagenicity test, revertant colonies did not exceed spontaneous colonies in number. Colony numbers did not increase in either strain after HO treatment, with or without metabolic activation. HO showed no mutagenic effects and did not induce a mutation in either strain. In the antimutagenicity test, HO reduced the number of mutated colonies induced by 4NQO in both strains. The inhibition rates of HO (TA98, 21-91%; TA100, 21-85%) indicated a potent reduction in mutagenicity induced by 4NQO. HO showed no significant mutagenicity and may have antimutagenic effects, as assessed by Ames testing.
목차
서론
재료 및 방법
재료
삼종실유
Gas chromatography-mass spectrometry analysis
총 플라보노이드 및 총 페놀화합물 함량측정
조직세포 배양
MTT assay
간조직 대사 활성제
Salmonella 돌연변이능 테스트
Salmonella 항돌연변이 테스트
통계 분석
결과 및 고찰
삼종실유의 지방산 함량
MTT assay
Ames test
감사의 글
문헌